The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry
✍ Scribed by Thomas, Carson H. ;McFarland, Clive D. ;Jenkins, Michelle L. ;Rezania, Alireza ;Steele, Jack G. ;Healy, Kevin E.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 399 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
In recent years a central objective of tissue engi-examined using light microscopy and digital image acquisineering has been understanding the interaction of cells with tion and subsequently were analyzed. Compared to complete biomaterial surfaces. In this study we examined the protein serum, the use of serum depleted of fibronectin with vitronecadsorption events necessary to control the attachment and tin included had minimal effect on the cell attachment, the subsequent spatial distribution of bone-derived cells ex-spreading, and spatial distribution on the EDS regions of the posed to chemically modified surfaces. Silane chemistry and surface. However, the use of serum depleted of vitronectin photolithography techniques were used to create substrates with or without fibronectin included resulted in greatly rewith alternating regions of an aminosilane, N-(2-amino-duced cell attachment and spreading. Thus the presence of ethyl)-3-aminopropyl-trimethoxysilane (EDS), along side an vitronectin was required for the attachment, spreading, and alkylsilane, dimethyldichlorosilane (DMS), on quartz sur-spatial distribution of bone-derived cells exposed to EDS/ faces. Sera depleted of fibronectin (Fn), vitronectin (Vn), or DMS-patterned surfaces.