The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

✍ Scribed by Sakurai, Yoshihiko; Otani, Yoshihide; Kameyama, Kaori; Igarashi, Naoki; Kubota, Tetsuro; Kumai, Koichiro; Kitajima, Masaki

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 272 KB
• Volume: 66
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/(sici)1096-9098(199711)66:3<168::aid-jso3>3.0.co;2-a

✦ Synopsis

Background and objectives:

In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. interstitial collagenase (matrix metalloproteinase-1: mmp-1) has been also investigated in relation to cancer invasion and metastasis.

Methods:

We investigated the formation and mechanism of mmp-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by elisa and immunohistochemistry.

Results:

Production of mmp-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of mkn-74. localization of mmp-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines.

Conclusions:

These results suggested that the production of mmp-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.