The role of specific lysine in cytochrome c3 on the electron transfer with hydrogenase

✍ Scribed by Noriyuki Asakura; Tomohiro Hiraishi; Toshiaki Kamachi; Ichiro Okura

• Book ID: 104421472
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 62 KB
• Volume: 174
• Category: Article
• ISSN: 1381-1169
• DOI: 10.1016/s1381-1169(01)00160-1

✦ Synopsis

Modification of specific lysine residue of cytochrome c 3 with 2,4,6-trinitorobenzenesulfonic acid sodium salt dehydrate (TNBS) was carried out. By comparing the hydrogen evolution rate with modified cytochrome c 3 and native cytochrome c 3 catalyzed by hydrogenase, the role of lysine residue of cytochrome c 3 was discussed. The modified lysine residue was responsible for the intermolecular electron transfer from cytochrome c 3 to hydrogenase.