The role of proteins in the nucleation and formation of calcium-containing deposits on biomaterial surfaces

✍ Scribed by Vasin, Sergei L. ;Rosanova, Irina B. ;Sevastianov, Viktor I.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 321 KB
• Volume: 39
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19980305)39:3<491::aid-jbm21>3.0.co;2-c

✦ Synopsis

In experiments in vivo using diffusion chambers, the morphology and composition of calcium-containing deposits on natural and artificial biomaterials that had no direct contact with cells were studied using scanning electron microscopy with energy dispersion X-ray microanalysis. It was revealed that the formation of a protein layer containing protein-calcium complexes is the key event in biomaterial calcification. A mechanism of formation of a calciumcontaining protein matrix that creates the conditions for su-persaturation of the crystal-forming medium over critical value has been proposed. The formation of nuclei of insoluble calcium phosphate starts predominantly deep in an adsorbed protein layer enriched by calcium ions.