𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The role of prostaglandin E1 in ornithine decarboxylase induction by tumor promoters

✍ Scribed by Alfred B. Ordman; R. C. Simsiman; Jeffrey S. Cleaveland; R. K. Boutwell

Publisher
John Wiley and Sons
Year
1986
Tongue
French
Weight
494 KB
Volume
37
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

The effect of topical application of PGE on induction of ODC in mouse epidermis was measured. When direct induction of ODC by TPA was blocked by also applying indomethacin, maximum ODC activity occurred only when PGE was applied simultaneously with TPA 4% hr before killing of the mice. If either TPA or PGE was applied at other times, ODC activity decreased substantially. Induction of ODC by mezerein was blocked by indomethacin but restored by PGE, as was observed with TPA, but induction by ethyl phenylpropiolate was not affected by indomethacin or PGE. DMBA did not cause a consistent increase in ODC activity, nor was its inductive action affected by indomethacin or PGE. However, another weak inducer, acetic acid, exhibited elevated ODC activity when PGE was also applied. Inhibition by topical retinoic acid of ODC induction by TPA was partially overcome in a dose-response fashion by PGE. The results indicate that at least 2 events, elevation of PGE and another independent event, are required for induction of ODC activity. It appears that TPA causes at least 4 independent events essential for tumor promotion. A model for the events in the 2-stage tumor promotion model is proposed.

'On leave from Dept. of BiolChemistry,

📜 SIMILAR VOLUMES

Core promoter involvement in the inducti
Core promoter involvement in the induction of rat ornithine decarboxylase by phorbol esters
✍ Biwei Zhao; Andrew P. Butler 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 151 KB

## Abstract Overexpression of ornithine decarboxylase (ODC) is an important oncogenic event in tumorigenesis. Although __ODC__ was one of the first genes described whose product is inducible by 12‐__O__‐tetradecanoylphorbol‐13‐acetate (TPA), the mechanisms of __ODC__ transcriptional regulation have

The role of cAMP in the induction of orn
The role of cAMP in the induction of ornithine decarboxylase by nerve and epidermal growth factors
✍ Monique Lacorbiere; David Schubert 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 386 KB

## Abstract Nerve growth factor (NGF), epidermal growth factor (EGF), adenosine 3′,5′‐cyclic monophosphate (cAMP), and cholera toxin all increase the specific activity of ornithine decarboxylase (ODC) in the PC12 nerve‐like cell line. A phosphodiesterase inhibitor augments the effect of NGF on the

Relation of the induction of epidermal o
Relation of the induction of epidermal ornithine decarboxylase and hyperplasia to the different skin tumor–promotion susceptibilities of protein kinase Cα, -δ and -ϵ transgenic mice
✍ Aaron P. Jansen; Nancy E. Dreckschmidt; Eric G. Verwiebe; Deric L. Wheeler; Terr 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 302 KB

To define the in vivo role of individual PKC isoforms in mouse skin carcinogenesis, we previously characterized FVB/n transgenic mice that over-expressed epitope-tagged PKC delta (T7-PKC delta) or PKC epsilon (T7-PKC epsilon) isoforms under the regulation of the human K14 promoter. In continuation o

Control of hepatocyte DNA synthesis by i
Control of hepatocyte DNA synthesis by intracellular pH and its role in the action of tumor promoters
✍ Chow H. Lee; Edward J. Cragoe Jr.; Anthony M. Edwards 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

## Abstract The mechanisms of tumor promotion in liver by various xenobiotics of diverse structure are not well understood. However, these tumor promoters share the ability to exert growth‐stimulatory effects on hepatocytes. Our laboratory has been utilizing normal rat hepatocytes under defined con

Preserved induction of long-term potenti
Preserved induction of long-term potentiation in the stratum radiatum in the CA1 field of hippocampal slices from transgenic mice overexpressing ornithine decarboxylase and overproducing putrescine
✍ R. Pussinen; J. Sirviö; L. Alhonen; J. Larson; M. Halmekytö; E. Koivisto; J. Jän 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 55 KB 👁 2 views

The role of putrescine in synaptic neurotransmission and plasticity was studied using transgenic mice overexpressing ornithine decarboxylase (ODC), a polyamine-synthesizing enzyme. Transgenic mice were produced using the standard microinjection technique leading to elevated levels of putrescine in t