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The role of p21cip1 in adaptation of CHO cells to suspension and protein-free culture

✍ Scribed by Kelly Astley; Mariam Naciri; Andrew Racher; Mohamed Al-Rubeai

Book ID
118471950
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
988 KB
Volume
130
Category
Article
ISSN
0168-1656

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## Abstract Chinese hamster ovary cells have been engineered to inducibly over‐express the p21^CIP1^ cyclin‐dependent kinase inhibitor, to achieve cell cycle arrest and increase cell productivity. In p21^CIP1^‐arrested cells production of antibody from a stably integrated lgG4 gene, was enhanced ap