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The role of misrepair processes in the isolation of new types of streptomycin-resistant mutants in Escherichia coli

✍ Scribed by Lacoste, Lucille ;Lacaille, Michel ;Brakier-Gingras, L�a

Publisher
Springer
Year
1977
Tongue
English
Weight
533 KB
Volume
157
Category
Article
ISSN
0026-8925

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✦ Synopsis

Treatment of Escherichia coli cells with ethylmethanesulfonate followed by a prolonged delay for phenotypic expression allows to select new types of streptomycin-resistant mutants, which are double mutants with one change resulting from base mispairing and the second one from misrepair. The error-prone recA-dependent pathway is involved in this misrepair, as evidenced by the fact that recA strains do not provide double mutants.

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