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The role of iron in the growth of human leukemic cell lines

โœ Scribed by Monique Titeux; Ugo Testa; Fawzia Louache; Pierre Thomopoulos; Henri Rochant; Jeanine Breton-Gorius

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
548 KB
Volume
121
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

The growth requirements of three human leukemic cell lines (K 562, HEL, U937) have been studied in the absence of serum. For growth in serum-free medium, the cells require insulin, transferrin, and albumin. Two highly watersoluble iron salts, ferric ammonium citrate and ferric ammonium sulfate, may completely replace transferrin for supporting the growth of these cell lines. Similar results were obtained when mitogen-stimulated lymphocytes were grown in serum-free media. Iron containing compounds, such as hemin or hemoglobin, were also able to replace transferrin. Experiments using 42/6 monoclonal antibody strongly suggest that free-iron salts are taken u p by the cells by a mechanism that is completely independent from transferrinreceptors.

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