𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The role of iron and glutathione in t-butyl hydroperoxide-induced damage towards isolated perfused rat livers

✍ Scribed by Maged Younes; Otfried Strubeit

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
505 KB
Volume
10
Category
Article
ISSN
0260-437X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The hepatotoxic and lipid peroxidative potentials of t‐butyl hydroperoxide (t‐BuOOH) towards isolated perfused rat livers were investigated at doses of 1 and 3 mmol I^−1^. t‐BuOOH led to a concentration‐dependent release of cytosolic (glutamate‐pyruvate transaminase and lactate dehydrogenase) and mitochondrial (glutamate dehydrogenase) enzymes, an accumulation of calcium in the liver, a marked depletion of hepatic glutathione and an enhanced release of it into the perfusate, as well as an enhanced formation and release of malondialdehyde (MDA) by the liver. These effects were blocked in the presence of the potent iron chelator deferrioxamine, and enhanced in livers from iron‐overloaded as well as in livers from glutathione‐depleted rats. Our results indicate that the hepatotoxic and pro‐oxidant actions of organic hydroperoxides depend upon the presence of ionized iron as a catalyst of radical‐forming breakdown reactions, and are potentiated by impairment of glutathione‐dependent detoxification reactions.

📜 SIMILAR VOLUMES

The role of iron in t-butyl hydroperoxid
The role of iron in t-butyl hydroperoxide-induced lipid peroxidation and hepatotoxicity in rats
✍ Maged Younes; Alexander Wess 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 404 KB 👁 1 views

## Abstract Treatment of rats with 100 mg kg^−1^ __t__‐butyl hydroperoxide led to an enhanced ethane exhalation as a marker of __in vivo__ lipid peroxidation, as well as a moderate hepatoxicity as evidenced by a rise in plasma activities of liver‐specific enzymes (glutamate‐pyruvate transaminase an