The role of glutathione in renal cortical tissue. effects of diamide on Na+and GSSG levels, amino acid transport and Na+-K+-ATPase activity

✍ Scribed by Dennis J. Pillion; Lamar Moree; Hector Rocha; David H. Pashley; Joseph Mendicino; Frederick H. Leibach

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 599 KB
• Volume: 18
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00280276

✦ Synopsis

The effects of diamide were studied in rat kidney cortical tissue. It was found that diamide increased oxidized glutathione levels and inhibited Na+-K+-ATPase activity. Consistent with this finding was the observation that diamide compromised the sodium gradients maintained in renal cortical slices. Amino acid transport studies with ouabain or a sodium-free buffer indicated that diamide interferes with both Na+-dependent and Na+-independent transport systems. These results indicate that diamide has a number of different effects on renal cortical tissue and emphasize the important role of glutathione in maintaining control of a number of key metabolic pathways.