The role of electron capture dissociation in biomolecular analysis

✍ Scribed by Helen J. Cooper; Kristina Håkansson; Alan G. Marshall

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 264 KB
• Volume: 24
• Category: Article
• ISSN: 0277-7037
• DOI: 10.1002/mas.20014

✦ Synopsis

Abstract

The introduction of electron capture dissociation (ECD) to electrospray (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR MS) constitutes a significant advance in the structural analysis of biomolecules. The fundamental features and benefits of ECD are discussed in this review. ECD is currently unique to FT‐ICR MS and the fundamentals of that technique are outlined. The advantages and complementarity of ECD in relation to other tandem mass spectrometry (MS/MS) techniques, such as infrared multiphoton dissociation (IRMPD) and sustained off‐resonance collision‐induced dissociation (SORI‐CID), are discussed. The instrumental considerations associated with implementation of ECD, including activated ion techniques and coupling to on‐line separation techniques, are covered, as are the allied processes electronic excitation dissociation (EED), electron detachment dissociation (EDD), and hot electron capture (HECD). A major theme of this review is the role of ECD in proteomics, particularly for characterization of post‐translational modifications (phosphorylation, glycosylation, carboxyglutamic acid, sulfation, acylation, and methionine oxidation) and the top‐down approach to protein identification. The application of ECD to the analysis of polymers, peptide nucleic acids, and oligonucleotides is also discussed. © 2004 Wiley Periodicals, Inc., Mass Spec Rev 24:201–222, 2005