The Role of Coherence Transfer Efficiency in Design of TROSY-Type Multidimensional NMR Experiments
✍ Scribed by Axel Meissner; Ole Winneche Sørensen
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 70 KB
- Volume
- 139
- Category
- Article
- ISSN
- 1090-7807
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✦ Synopsis
An improved method for TROSY-type (Pervushin et al., Proc. Natl. Acad. Sci. USA 94, 12366-12371 (1997)) heteronuclear two-dimensional correlation involving protons of negligible CSA is presented. Rather than applying a simple INEPT sequence for back-transfer to protons (Pervushin et al., J. Am. Chem. Soc. 120, 6394-6400 (1998)), we replace the pi/2 proton pulse in INEPT by a spin-state-selective coherence transfer element (Sorensen et al., J. Biomol. NMR 10, 181-186 (1997)) and maintain broadband decoupling during acquisition. Theoretically that results in a sensitivity enhancement of a factor of 2. The new method is demonstrated using a (13)C,(15)N-labeled protein sample, RAP 18-112 (N-terminal domain of alpha(2)-macroglobulin receptor associated protein), at 750 MHz.