The role of calcium ions in the permeability changes produced by external ATP in transformed 3T3 cells
β Scribed by Barun K. De; Gary A. Weisman
- Book ID
- 115726603
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 553 KB
- Volume
- 775
- Category
- Article
- ISSN
- 0005-2736
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## Abstract Exogenous ATP has been shown earlier to activate a permeability change in transformed 3T3 cultures leading to massive efflux of the acidsoluble pools. This leads to reduction of the basal rate of glycolysis to a very low level so that glycolysis becomes almost totally dependent on the a
## Abstract Balb/3T3 cells transformed in culture by chemical carcinogens were shown to multiply in a medium supplemented with 2% calf serum or with 10% agamma newβborn calf serum. The cell lines that multiply well in medium supplemented with 10% agamma serum produced a higher incidence of tumors i
## Abstract Glucocorticosteroids, when added two hours after cell plating to SV40βtransformed, 3T3 mouse fibroblasts in low serum (0.3%v/v), biotinβsupplemented medium, suppress cellular proliferation by 24 hours. While some cell death probably occurs, the growth inhibition is not primarily due to
## Abstract Addition of ATP to medium surrounding intact, transformed 3T3 cells activates the formation of aqueous channels in the plasma membrane. This results in efflux of nucleotide pools and ions and entry into the cytosol of charged, phosphorylated species. In such permeabilized cells, glycoly