✦ LIBER ✦

The role of anion exchange in the uptake of Pb by human erythrocytes and Madin-Darby canine kidney cells

✍ Scribed by Desmond I. Bannon; Luisa Olivi; Joseph Bressler

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 92 KB
Volume: 147
Category: Article
ISSN: 0300-483X
DOI: 10.1016/s0300-483x(00)00187-6

No coin nor oath required. For personal study only.

✦ Synopsis

Anion exchange (AE) plays a critical role in regulating intracellular pH in erythrocytes and epithelial cells and has been suggested to facilitate the transport of lead (Pb) across the erythrocyte cell membrane. In this study we examined the role of AE in the uptake of Pb by human erythrocytes and by Madin-Darby canine kidney (MDCK) cells, the kidney epithelial cell line. Functional AE in MDCK cells was evidenced by: increased uptake of SO 4 2at pH 6.0 over pH 7.0, and inhibition of SO 4 2uptake by the AE inhibitor 4, 4%-diisothiocyanostilbene-2, 2%-disulfonic acid (DIDS) as well as by non-halide anions. Accumulation of Pb into MDCK cells was time and temperature dependent. DIDS inhibited uptake of Pb into human erythrocytes but not MDCK cells. In conclusion, uptake of Pb into erythrocytes but not kidney epithelial cells occurs through AE.

📜 SIMILAR VOLUMES

Role of anion exchange and thiol groups

Role of anion exchange and thiol groups in the regulation of potassium efflux by lead in human erythrocytes

✍ Bachchu Lal; Gary Goldstein; Joseph P. Bressler 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 1002 KB

Pb" is thought to enter erythrocytes through anion exchange (AE) and to remain in the cell by binding to thiol groups. To define the role of AE mechanism and thiol groups in Pb2+ toxicity, we studied the effects c f drugs and conditions that modify AE and that modify thiol groups on the ability of P