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The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples

✍ Scribed by Hideaki Obara; Naoki Aikawa; Naoki Hasegawa; Shingo Hori; Yasuo Ikeda; Yoshio Kobayashi; Mitsuru Murata; Shinichiro Okamoto; Junzo Takeda; Minoru Tanabe; Yasuhiko Sakakura; Hiroyuki Ginba; Masaki Kitajima; Yuko Kitagawa


Publisher
Springer
Year
2011
Tongue
English
Weight
196 KB
Volume
17
Category
Article
ISSN
1341-321X

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## Abstract A two‐step reverse transcriptase TaqMan™ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific