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The reversibility of N-ethylmaleimide (NEM) alkylation of red cell glutathione

✍ Scribed by Ernest Beutler; Satish K. Srivastava; Carol West

Book ID
118852040
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
315 KB
Volume
38
Category
Article
ISSN
0006-291X

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πŸ“œ SIMILAR VOLUMES

Assay of thiols and disulfides based on
Assay of thiols and disulfides based on the reversibility of N-ethylmaleimide alkylation of thiols combined with electrolysis
✍ Junko Nishiyama; Toyo Kuninori πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 544 KB

A simple and specific method for analyzing thiols and disulfides on the basis of the reversibility of N-ethylmaleimide (NEM) alkylation of thiols is described. When the adduct of NEM and glutathione (GSH) was electrolyzed at neutral pH, all of the GSH was recovered. When the adduct was exposed to pH