The response of germ cells to ethylene oxide, propylene oxide, propylene imine and methyl methanesulfonate is a matter of cell stage-related DNA repair
✍ Scribed by Ekkehart W. Vogel; Madeleine J. Nivard
- Book ID
- 101264448
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 128 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
We describe the consequences of a defect for nucle-a hypermutability, which, on the average, was 2.4otide excision repair (NER) in oocytes for alkylation-fold lower than for EO. This indicates that the 2induced mutagenesis in different germ-cell stages of hydroxyethyl adduct generated by EO is more effi-Drosophila males. Mutant frequencies induced in ciently repaired than the 2-hydroxypropyl adduct NER / condition (cross NER / && 1 NER / () were caused by PO. The low SLRL frequencies (0.2compared with those fixed in a NER 0 background 0.9%) estimated for propylene imine (PI) in NER / (cross NER 0 && 1 NER / (), using the X-linked re-genotypes showed no relation to dose in the range cessive lethal assay (SLRL) for the measurement of from 1,500 to 48,000 ppmrh. In the absence of forward mutations in 700 loci. In successive male NER, mutant frequencies were increased up to 29germ-cell stages exposed to a low dose of 2.4 fold, and a dose-dependent increase in mutations mMrh methyl methanesulfonate, efficient repair of was observed for PI over the entire dose range. This premutational damage in spermatogonia and by study shows mutation induction by EO in postmeithe maternal repair system after fertilization was otic Drosophila germ cells at exposure doses that observed. Ethylene oxide (EO) and propylene oxide are 800-fold below those applied previously in the (PO) did not induce high mutant frequencies in post-mouse specific-locus test on spermatogonia [with meiotic germ cells when mutagenized males were negative response; Russell et al. (1984): Mutat Res mated with NER / females: a 32-fold increase in 129:381-388] and 11-fold below the EO dose dose from 750 ppmrh to 24,000 ppmrh EO for which increased dominant-lethal responses and (ÉLD 50 ) led to no more than a 3-fold enhancement heritable translocations were documented in mice in mutant frequency. However, up to a 17-fold in-spermatozoa and spermatids [Generoso et al. crease in mutant frequencies were obtained with (1990): Environ Mol Mutagen 16:126 -131]. Envi-NER 0 females. In matings with NER / females, PO ron. Mol. Mutagen. 29:124-135, 1997 was about 10 times less mutagenic than EO. Sup-᭧ 1997 Wiley-Liss, Inc. pression of the maternal NER system caused