The response of different genotypes ofFragaria × ananassaand their seedling progenies to in vitro micropropagation and the effects of varying the concentration of 6-benzylaminopurine in the proliferation medium
✍ Scribed by David W. Simpson; Judith A. Bell
- Book ID
- 104622488
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 464 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Two experiments are reported. In the first, six different strawberry genotypes were each grown in vitro on media containing five different concentrations of 6-benzylaminopurine (BAP) ranging from 0.5#M to 8.0/~M. Responses of the genotypes to different BAP levels varied and the optimum levels for maximum proliferation were between 1/aM and 8#M. In the second experiment the six genotypes were split into two groups of three and all crosses between the two groups were made. Seedlings from the nine progenies were then grown on media with the same five levels of BAP. Of the 12% of seedlings that did not proliferate, most were on media with the two lowest BAP levels (0.5 and 1.0/~M). Seedlings from crosses involving the cv. Bogota proliferated least, while the three progenies with the breeding line ES652 as a parent had the highest mean proliferation rates. One cross, Bogota × Providence, had a particularly low mean proliferation rate. It was concluded that to optimize proliferation rate the medium must be tailored to the genotype. The best medium could not be reliably predicted from knowledge of the pedigree of a genotype.