The Resolution Revolution: Recent Advances In cryo: EM

✍ Scribed by R.A. Crowther (Eds.)

• Publisher: Academic Press
• Year: 2016
• Tongue: English
• Leaves: 450
• Series: Methods in Enzymology 579
• Edition: 1
• Category: Library

✦ Synopsis

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods that determine the structures of biological molecules, a vital step for understanding their function
• Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

✦ Table of Contents

Content:
Series Page Page ii
Copyright Page iv
Contributors Pages xi-xii
Preface Pages xiii-xx R.A. Crowther
Chapter One - Direct Electron Detectors Pages 1-17 G. McMullan, A.R. Faruqi, R. Henderson
Chapter Two - Specimen Behavior in the Electron Beam Pages 19-50 R.M. Glaeser
Chapter Three - Specimen Preparation for High-Resolution Cryo-EM Pages 51-86 L.A. Passmore, C.J. Russo
Chapter Four - Strategies for Automated CryoEM Data Collection Using Direct Detectors Pages 87-102 A. Cheng, Y.Z. Tan, V.P. Dandey, C.S. Potter, B. Carragher
Chapter Five - Processing of Cryo-EM Movie Data Pages 103-124 Z.A. Ripstein, J.L. Rubinstein
Chapter Six - Processing of Structurally Heterogeneous Cryo-EM Data in RELION Pages 125-157 S.H.W. Scheres
Chapter Seven - Single-Particle Refinement and Variability Analysis in EMAN2.1 Pages 159-189 S.J. Ludtke
Chapter Eight - Frealign: An Exploratory Tool for Single-Particle Cryo-EM Pages 191-226 N. Grigorieff
Chapter Nine - Testing the Validity of Single-Particle Maps at Low and High Resolution Pages 227-253 P.B. Rosenthal
Chapter Ten - Tools for Model Building and Optimization into Near-Atomic Resolution Electron Cryo-Microscopy Density Maps Pages 255-276 F. DiMaio, W. Chiu
Chapter Eleven - Refinement of Atomic Structures Against cryo-EM Maps Pages 277-305 G.N. Murshudov
Chapter Twelve - Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction Pages 307-328 S.A. Fromm, C. Sachse
Chapter Thirteen - Cryo-Electron Tomography and Subtomogram Averaging Pages 329-367 W. Wan, J.A.G. Briggs
Chapter Fourteen - High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method Pages 369-392 J.A. Rodriguez, T. Gonen
Chapter Fifteen - Databases and Archiving for CryoEM Pages 393-412 A. Patwardhan, C.L. Lawson
Author Index Pages 413-429
Subject Index Pages 431-445