The requirement of low pH for growth of Thiobacillus thiooxidans

✍ Scribed by Rao, G. Sivaji ;Berger, Leslie R.

• Book ID: 104769887
• Publisher: Springer-Verlag
• Year: 1971
• Weight: 380 KB
• Volume: 79
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00424909

✦ Synopsis

Thiobacillus thiooxidans was grown at constant pH. No growth was obtained above pH 4.3. Sulfur was oxidized over the entire range tested (pH 0.9 to 7.0). This was confirmed by pH shift experiments. Carbon dioxide fixation stopped 30 min after shift from low pI-I to neutral values. On return to high acidity, the rate of COn assimilation increased but not always to the original value. The intracellular binding of glycerol which is passively permeable was also inhibited by raising the ptI of the medium. The data suggested that ATP formation may be inhibited at neutral pH. Intraccllular ATP pool was reduced by 80~ on neutralization of the acid growth medium. These results are compared to recent studies