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The replication of Escherichia coli chromosome can be initiated by integrated ColE1-type plasmids

โœ Scribed by Strizhov, N. I. ;Tikhomirova, L. P. ;Bayev, A. A.

Publisher
Springer
Year
1981
Tongue
English
Weight
307 KB
Volume
182
Category
Article
ISSN
0026-8925

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recA Gene dependence of replication of t
recA Gene dependence of replication of the Escherichia coli chromosome initiated by plasmid pUC13 integrated at predetermined sites
โœ Mao, Yu-Min ;Shi, Qin ;Li, Qing-Ge ;Sheng, Zu-Jia ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 827 KB

Plasmid pUC13 was used to clone DNA fragments of known sites from the chromosome of Escherichia coli. Each chimeric plasmid was introduced individually into the same dnaA46 mutant strain LC381 and suppressive integration (Sin) strains were selected. By means of cotransduction the null mutation recA5