𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The relevance of pH gradients in immobilized enzymes

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
68 KB
Volume
106
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Quantitative evaluation of protein recov
Quantitative evaluation of protein recoveries in two-dimensional electrophoresis with immobilized pH gradients
✍ Xun Zuo; David W. Speicher πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 208 KB πŸ‘ 1 views

In this study, metabolically radiolabeled Escherichia coli cell extracts were used to systematically evaluate protein recoveries at each step of two-dimensional (2-D) electrophoresis and using different sample application methods. Sample application using sample cups resulted in better protein recov

A mathematical model for the generation
A mathematical model for the generation and control of a pH gradient in an immobilized enzyme system involving acid generation
✍ Guodong Chen; Ronald L. Fournier; Sasidhar Varanasi πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 345 KB πŸ‘ 2 views

An optimal pH control technique has been developed for multistep enzymatic synthesis reactions where the optimal pH differs by several units for each step. This technique separates an acidic environment from a basic environment by the hydrolysis of urea within a thin layer of immobilized urease. Wit

Modified immobilized pH gradient gel str
Modified immobilized pH gradient gel strip equilibration procedure in SWISS-2DPAGE protocols
✍ Jun X. Yan; Jean-Charles Sanchez; Veronique Rouge; Keith L. Williams; Denis F. H πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 144 KB

## Modified immobilized pH gradient gel strip equilibration procedure in SWISS-2DPAGE protocols In the present paper we report a revised protocol for immobilized pH gradient (IPG) gel strip equilibration involving a procedural modification between the first-and seconddimensional separation in both