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The relationship between mRNA half-life and gene function in the yeast Saccharomyces cerevisiae

✍ Scribed by Joyce Moore; Howard T. Jacobs; Kim Kaiser

Book ID
116133156
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
431 KB
Volume
166
Category
Article
ISSN
0378-1119

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The decay kinetics of mRNA was studied in a yeast temperature-sensitive mutant, ts136, which is defective in cytoplasmic RNA production at 37 degree C. The disappearance of the synthetic capacity of mRNA was determined by withdrawing equal volumes of ts136 cell culture and pulse-labelling with [35S]