The relationship between molecular size and electrophoretic mobility in agarose gels as determined from a single population of growing RNA molecules
✍ Scribed by K. Andersson; M. Fagerlind; B. Daneholt
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 398 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0301-4851
No coin nor oath required. For personal study only.
✦ Synopsis
Growing RNA molecules obtained from a defined transcription unit, the 75 S RNA transcription unit in Balbiani ring 2 of Chironomus tentans, have been analysed by electrophoresis in agarose. This set of RNA molecules forms a broad distribution of molecules with sizes ranging from very small up to the final product of giant size corresponding to 75 S RNA. The relative molecular weights along the migration path were calculated from a simple activity relationship derived from the expected distribution properties of a single population of growing RNA molecules. It was concluded that there is a linear relationship between the molecular weight and the logarithm for the mobility.