The relationship between hydrogen gas and butanol production by Clostridium saccharoperbutylacetonicum

✍ Scribed by James D. Brosseau; Jwo-Yee Yan; K. Victor Lo

• Book ID: 101723606
• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 457 KB
• Volume: 28
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260280302

✦ Synopsis

Two simultaneous fermentations were performed at 26°C with simultaneous inocula using Clostridium saccharoperbotylacetonicurn. Fermentation 1 prevented the gas formed by the biomass from escaping the fermentor while 2 allowed the gas formed to escape. Fermentor 1 provided for the production of butanol, acetone, and ethanol, while when the H2 formed was allowed to escape with fermentor 2, neither butanol nor acetone were produced. Ethanol was also formed in both fermentors and began along with the initial growth of biomass and continued until the fermentations were complete. Butanol and acetone production began after biomass growth had reached a maximum and began to subside. The butanolacetone-ethanol millimolar yields and ratios were 38: 1 : 14 respectively. The fermentor 2 results show that a yield of 2.1 L H2, 93 or 370 mmol H,/mol glucose, was formed only during the growing stage of growth; neither butanol nor acetone were produced; ethanol was formed throughout the fermentation, reaching a yield of 15.2 mmolar. It appears that hydrogen gas is required for butanol production during the resting stage of growth.