Mammalian oocytes exhibit a series of cell cycle transitions that coordinate the penultimate events of meiosis with the onset of embryogenesis at fertilization. The execution of these cell cycle transitions, at G& of meiosis-I and metaphase/anaphase of meiosis I and 11, involve both biosynthetic and
The regulation of maturation promoting factor during prophase I arrest and meiotic entry in mammalian oocytes
β Scribed by Adhikari, Deepak; Liu, Kui
- Book ID
- 122407404
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 775 KB
- Volume
- 382
- Category
- Article
- ISSN
- 0303-7207
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## Abstract Cell fusions have been used to determine the biological activity of the MPF complex in murine oocytes during their progression through anaphase and telophase to metaphase II. Oocytes (1) at metaphase I, (2) during the anaphaseβtelophase transition, or (3) at metaphase II were fused to g
The present study was carried out using the method of electrofusion, or treatment with okadaic acid (OA), to determine whether protein synthesis at the onset of culture was required for the meiotic resumption of bovine follicular oocytes. Germinal vesicle breakdown (GVBD) occurred in bovine oocytes