𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The recovery of staphylococcal enterotoxin from bacterial culture supernatants by ion exchange

✍ Scribed by Bergdoll, M. S. ;Sugiyama, H. ;Dack, G. M.

Book ID
102302815
Publisher
Wiley (John Wiley & Sons)
Year
1961
Weight
457 KB
Volume
3
Category
Article
ISSN
0368-1467

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✦ Synopsis

Abstract

A method has been developed for the recovery of enterotoxin from bacterial culture supernatants of staphylococcus strain S‐6 by use of the ion‐exchange resin Amberlite XE‐64 (IRC‐50). The enterotoxin from 200 ml of bacterial culture supernatant diluted with one volume of water and adjusted to pH 6 can be removed with 1 g of resin pretreated with 0·02 M sodium phosphate, pH 6·2. The enterotoxin is recovered from the resin with 0·2 M sodium phosphate, pH 6·2, with subsequent precipitation with EtOH at −10°C, dialysis, and freeze drying. Partially purified preparations containing 1 emetic dose per 50μg were obtained.

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