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The Reaction Process of Firefly Bioluminescence Triggered by Photolysis of Caged-ATP

✍ Scribed by Takeshi Kageyama; Masatoshi Tanaka; Takao Sekiya; Shin-Ya Ohno; Naohisa Wada


Book ID
111413721
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
338 KB
Volume
87
Category
Article
ISSN
0031-8655

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A highly sensitive ATP bioluminescence assay with diethylaminoethyl-dextran (DEAE-Dx) in the presence of ATP extractants such as trichloroacetic acid (TCA) and Triton X-100 is described. These ATP extractants inhibited the activity of firefly luciferase, resulting in a remarkable decrease in the int