The rate of oxygen consumption and anaerobic glycolysis of the nuclei of chicken erythrocytes

✍ Scribed by Hunter, F. R. ;Banfield, W. G.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1943
• Tongue: English
• Weight: 92 KB
• Volume: 22
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030220309

✦ Synopsis

Laskowski ('42) described a method for obtaining nuclei from hens' erythrocytes using lysolecithin and presented preliminary data for their rate of oxygen consumption. Dounce and Lan ( '43) presented a similar method for obtaining nuclei from erythrocytes using saponin which gave slightly higher values for oxygen consumption. Some time ago in this laboratory nuclei were obtained from chicken erythrocytes using a method very similar to that of Dounce and Lan ( '43). Values for oxygen consumption and for anaerobic glycolysis are presented in tables 1 and 2. Some nuclei were obtained merely by hemolyzing erythrocytes in water. Others were obtained using a solution of saponin in water while still others were obtained using a solution of saponin in Ringer-Locke.