The rat prostatic epithelial cell line NRP-152 can differentiate in vivo in response to its stromal environment

✍ Scribed by Hayward, Simon W.; Haughney, Peter C.; Lopes, Erin S.; Danielpour, David; Cunha, Gerald R.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 792 KB
• Volume: 39
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(19990515)39:3<205::aid-pros9>3.0.co;2-m

✦ Synopsis

Background:

The clonally derived rat prostatic epithelial cell line nrp-152 was examined to determine its ability to differentiate in a tissue recombination model.

Methods:

Nrp-152 cells alone, or combined with urogenital mesenchyme (ugm) or 10t1/2 fibroblasts, were grafted beneath the renal capsule of athymic rodent hosts. after 1 and 3 months, grafts were examined grossly and immunohistochemically.

Results:

Nrp-152 cells grafted alone formed small (10-25 mg) grafts without recognizable architecture. nrp-152 cells recombined with ugm formed larger grafts (50-100 mg after 28 days) containing glandular epithelium. columnar luminal cells expressed cytokeratins 8 and 18 and rat prostatic secretory markers (dp-1 and dp-2). the epithelial ducts were surrounded by well-differentiated smooth muscle. the glandular epithelial cells were shown to be of rat origin. nrp-152 + 10t1/2 tissue recombinants formed small grafts (10-40 mg wet weight) after 1 month. the epithelial component of these grafts formed solid unbranched cords expressing cytokeratins 5 and 14; no glandular epithelial structures were observed. the stromal matrix was densely packed with a few cells expressing alpha-actin.

Conclusions:

A clonally derived prostatic epithelial cell line can form structurally and functionally normal prostatic tissue. this suggests that prostatic basal and luminal epithelial cells can be derived from a common progenitor.