The RAD6 DNA repair pathway in Saccharomyces cerevisiae: What does it do, and how does it do it?

✍ Scribed by Christopher Lawrence

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 729 KB
• Volume: 16
• Category: Article
• ISSN: 0265-9247
• DOI: 10.1002/bies.950160408

✦ Synopsis

The RAD6 pathway of budding yeast, Saccharomyces cerevisiae, is responsible for a substantial fraction of this organism's resistance to DNA damage, and also for induced mutagenesis. The pathway appears to incorporate two different recovery processes, both regulated by RAD6. The error-prone recovery process accounts for only a small amount of RAD6-dependent resistance, but probably all induced mutagenesis. The underlying mechanism for error-prone recovery is very likely to be translesion synthesis. The error-free recovery process accounts for most of RAD6-dependent resistance, but its mechanism is less clear; it may entail error-free bypass by template switching and/or DNA gap filling by recombination. RAD6 regulates these activities by ubiquitinating target proteins, but the identities of these target proteins, and the roles they play in error-free and error-prone recovery, have not yet been established. 2. Error-free recovery RADS 134 RAD9 148 RAD18 55 No No No No Yes Yes No No No E2 Ubiquitin-conjugating Homology with E. coli umuC protein(36) DNA polymerase?(34) ? Protein kina~e(3~) Thymidylate kinasd40) DNA helicase? binds DNA?(23) ?(43) Binds DNA? A T P ~s ~? ( * ' * ~' )