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The quantitative determination of bile salts in bile using thin-layer chromatography and 3α-hydroxysteroid dehydrogenase

✍ Scribed by Leslie A. Turnberg; Alethea Anthony-Mote

Book ID
115818412
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
473 KB
Volume
24
Category
Article
ISSN
0009-8981

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A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol