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The purification of SP1-β (pregnancy-specific β1-glycoprotein) using immobilized triazine dyes

✍ Scribed by Kevin G. McFarthing; Sarojani Angal; Peter D.G. Dean

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
761 KB
Volume
122
Category
Article
ISSN
0003-2697

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✦ Synopsis

Pregnancy-specific &glycoprotein was purified to 99% purity by sequential chromatography on carboxymethyl-cellulose (to remove other pregnancy-specific &-glycoprotein variants), Cibacron blue 3G-A-Matrex Gel, Procion turquoise MX-G-Matrex Gel, Procion red H-IBN-Matrex Gel, and rabbit anti-normal human male serum-Matrex Gel. The purification was rapid, large-scale, and gave a yield of pregnancy-specific /I,-glycoprotein of 35. I%.

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