An integrated downstream processing stra
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L P Fonseca; J M S Cabral
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Article
📅
2002
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Wiley (John Wiley & Sons)
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English
⚖ 301 KB
## Abstract An integrated process strategy for the recovery of penicillin acylase was developed, based on precipitation of non‐enzymatic proteins directly from __Escherichia coli__ homogenates or crude extracts using Rolquat (quaternary ammonium salt) and adsorption of the enzyme on Amp‐Seph (3.8 µ