THE PURIFICATION AND PROPERTIES OF RAT BRAIN GLUTAMATE DEHYDROGENASE
โ Scribed by Po Yok Chee; June L. Dahl; Leonard A. Fahien
- Book ID
- 111170779
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 510 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
As an initial step in our study of nitrogen metabolism in the coral/algal symbiosis we have purified glutamate dehydrogenase (EC 1.4.1.4) to homogeneity from polyp tissue of the staghorn coral Acropora formosa collected from Magnetic Island (North Queensland) in 1985-1986. The purified enzyme had a
NADP-dependent glutamate dehydrogenase from Dictyostelium discoideum was purified 9300 fold with a yield of 4.6%. The enzyme is a hexamer of apparent molecular weight 294 kDa on Sephacryl S400 and a subunit molecular weight of 52 kDa as determined by SDS gel electrophoresis. The apparent Kms for alp