The Proteomics Protocols Handbook || Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis
β Scribed by Walker, John M.
- Book ID
- 121342788
- Publisher
- Humana Press
- Year
- 2005
- Weight
- 36 KB
- Category
- Article
- ISBN
- 1592598900
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This paper investigates the preparation of Fasciola hepatica samples for two-dimensional electrophoresis (2-DE). Whole samples were prepared by both hot sodium dodecyl sulfate (SDS) solubilisation and precipitation using trichloroacetic acid (TCA) to remove nonprotein contaminants and to inactivate
## Abstract The present study reports a comparison of recently described solubilizing methods, to set up a simple protocol for obtaining twoβdimensional (2βD) gel electrophoresis maps of brain tissue. Different protocols were used for preparing rat brain homogenates and the resulting maps were comp