✦ LIBER ✦
The prooligonucleotide approach IV : Synthesis of chimeric prooligonucleotides with 6 enzymolabile masking groups and unexpected desulfurization side reaction
✍ Scribed by Guilhem Tosquellas; François Morvan; Bernard Rayner; Jean-Louis Imbach
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 264 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
✦ Synopsis
Chimeric dodecanucleotides exhibiting a central gap of 6 phosphorothioate internucleoside linkages masked with pivaloyloxymethyl groups and uncharged or charged flanks were synthesized by alkylation of the corresponding phosphorothioate oligos. In total CEM cell extract, they selectively yielded the unmasked charged oligos with a half-time of about 30 hours. Furthermore during alkylation of phosphorothioate linkages a partial desulfurization was observed.