The Production of Monoclonal Antibody in Growth-Arrested Hybridomas Cultivated in Suspension and Immobilized Modes
β Scribed by Douglas B. Seifert; Janice A. Phillips
- Book ID
- 109388256
- Publisher
- American Institute of Chemical Engineers
- Year
- 1999
- Tongue
- English
- Weight
- 182 KB
- Volume
- 15
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10Β°/0 FCS), giving K,,, = 1.6 v/v% a
The iron-rich (500 gM ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved ox