The production of long-term rat muscle cell cultures on a Matrigel substrate and the removal of fibroblast contamination by collagenase

High and low affinity binding sites for

High and low affinity binding sites for basic fibroblast growth factor on cultured cells: Absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cells

✍ David Moscatelli 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind