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The production of anti-hexapeptide antibodies which recognize the S7, L6 and L13 ribosomal proteins of Escherichia coli

✍ Scribed by Aristomenis Metaxas; Socrates Tzartos; Dr Maria Liakopoulou-Kyriakides

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
93 KB
Volume
8
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

Here we report the synthesis of the N‐terminal hexapeptide H‐Pro‐Arg‐Arg‐Arg‐Val‐Ile‐OH of the E. coli ribosomal protein S7, the C‐terminal hexapeptide H‐Lys‐Glu‐Ala‐Lys‐Lys‐Lys‐OH of L6 and the C‐terminal hexapeptide H‐Pro‐Gln‐Val‐Leu‐Asp‐Ile‐OH of L13. All peptides were prepared by SPPS following the Fmoc‐strategy, using DIC/HOBt and/or HBTU as coupling reagents and 2‐chlorotrityl chloride resin as the solid support. The carrier linked synthetic peptides were injected into rabbits and elicited an anti‐peptide response. These anti‐hexapeptide antibodies were found to recognize the corresponding peptides and proteins. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

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