𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The production and utilization of nitric oxide by a new, denitrifying strain ofPseudomonas aeruginosa

✍ Scribed by R. Voßwinkel; I. Neidt; H. Bothe

Publisher
Springer
Year
1991
Tongue
English
Weight
854 KB
Volume
156
Category
Article
ISSN
0302-8933

No coin nor oath required. For personal study only.

✦ Synopsis

When a new strain of Pseudomonas aeruginosa was grown aerobically and then transferred to anaerobic conditions, cells reduced NO~-quantitatively to NO2 in NO~-respiration. In the absence of nitrate, NO2 was immediately reduced to NO or NzO but not to N2 indicating that NOz-reductase but not NzO-reductase was active. The formation of the products NO or NzO depended on the pH in the medium and the concentration of NO2 present. When P. aeruginosa was grown anaerobically for at least three days NzO-reductase was also active. Such cells reduced NO to N2 via N20. The new strain generated a H+-gradient and grew by reducing NzO to N2 but not by converting NO to NzO. For comparison, Azospirillum brasilense Sp7 showed the same pattern of NO-reduction. In contrast, Paracoccus denitrificans formed 3.5 H+/NO during the reduction of NO to N20 in oxidant pulse experiments but could not grow in the presence of NO. Thus the NO-reduction pattern in P. denitrificans on one side and P. aeruginosa and A. brasilense on the other was very different. The mechanistic implications of such differences are discussed.

📜 SIMILAR VOLUMES

ChemInform Abstract: Javaberine A, New T
ChemInform Abstract: Javaberine A, New TNF-α and Nitric Oxide Production Inhibitor, from the Roots of Talinum paniculatum.
✍ Hiroshi Shimoda; Norihisa Nishida; Kiyofumi Ninomiya; Hisashi Matsuda; Masayuki 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 27 KB 👁 1 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

TGF-β1 potentiates astrocytic nitric oxi
TGF-β1 potentiates astrocytic nitric oxide production by expanding the population of astrocytes that express NOS-2
✍ Mary E. Hamby; James A. Hewett; Sandra J. Hewett 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 830 KB

## Abstract Both transforming growth factor‐β1 (TGF‐β1) and nitric oxide synthase‐2 (NOS‐2) are upregulated under various neuropathological states. Evidence suggests that TGF‐β1 can either attenuate or augment NOS‐2 expression, with the prevailing effect dependent on the experimental paradigm emplo

Time- and concentration-dependent produc
Time- and concentration-dependent production of superoxide anion, nitric oxide, DNA damage and cellular death by ricin in the J774A.1 macrophage cells
✍ Ezdihar A. Hassoun; Xin Wang 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 112 KB 👁 2 views

The time-and concentration-dependent effects of ricin on some biomarkers of cellular toxicity, including production of superoxide anion ( ), nitric ‫מ‬ O 2 oxide (NO), and DNA single strand breaks (SSB), as well as cellular death, have been examined in the J774A.1 macrophage cell cultures. Various c

Inhibition of endotoxin-induced nitric o
Inhibition of endotoxin-induced nitric oxide synthase production in microglial cells by the presence of astroglial cells: A role for transforming growth factor β
✍ V.A.M. Vincent; F.J.H. Tilders; A-M. Van Dam 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB 👁 2 views

In mixed glial cell cultures from cerebral cortices of newborn rats, endotoxin induces inducible nitric oxide (iNOS), nitric oxide (NO), and interleukin-1 beta (IL-1 beta) production in microglial cells. Earlier we demonstrated that endotoxin induced iNOS but not IL-1 beta expression in microglial c