The Problem of Nucleolar Number in Cell Nuclei and in Sections of Cell Nuclei

✍ Scribed by Dr. sc. nat. K. Voss; K. Roth

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 492 KB
• Volume: 27
• Category: Article
• ISSN: 0323-3847
• DOI: 10.1002/bimj.4710270215

✦ Synopsis

In thie peper the mean number m of nucleoli of cell nuclei and the relative frequency pN of cell nuclei with N nucleoli are inveatigated. To solve the firat problem, the stereologicel model of convex shaped nuclei and nucleoli and the esenmption of randomly-isotropically distributed objecta in space are ueed. The model is developed for non-vanishing thickness T of tieeue sections. The relationship between the unknown number m of nucleoli in oell nuclei and the mean number ii of nucleoli in nuclear aections determined by counting is R =ii (D + T)/(d + T). Here D and d are the mean va~ue-9 of caliper diameter for nuclei and for nucleoli, reap. The second problem is i~~ustrated by means of wme biomedical examplea: The relative frequency p~ of cell nuclei with N nucleoli can be approximated by a generalized Pokeon distribution in all investigated cases. Therefore the mean nucleolar number h l is the eS.Sfntia1 parameter to describe the frequencies of cell nuclei with different numbere of nucleoli