𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The primary structure of the basic isoform of Acanthamoeba profilin

✍ Scribed by Christophe AMPE; Masahiko SATO; Thomas D. POLLARD; Joël VANDEKERCKHOVE


Book ID
115125528
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
430 KB
Volume
170
Category
Article
ISSN
1432-1327

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Evaluation of the binding of Acanthamoeb
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We have used a fluorescence assay to measure the binding of Acanthamoeba profilin to monomeric Acanthamoeba and rabbit skeletal muscle actin labeled on cysteine-374 with pyrene iodoacetamide. The wavelengths of the pyrene excitation and emission maxima are constant at 346 and 386 nm, but the fluores