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The presumptive detection of some antibiotics in foods, using a simple microbiological test

✍ Scribed by L. P. van der Mijll Dekker; D. A. A. Mossel; A. S. de Bruin; A. Manten


Publisher
John Wiley and Sons
Year
1959
Tongue
English
Weight
346 KB
Volume
10
Category
Article
ISSN
0022-5142

No coin nor oath required. For personal study only.

✦ Synopsis


Abstract

A simple and sufficiently sensitive test for the presumptive detection of antibiotics in foods has been developed, based on the principle that the food under investigation is mixed with double‐strength agar containing triphenyltetrazolium chloride as an indicator of bacterial growth. The agar dispersion so obtained is streak‐inoculated with suitable test strains and incubated, using as a blank a mixture of agar and an identical food sample known to be free from added antibiotics.

For the presumptive detection of antibiotics Staphylococcus aureus and Streptococcus cremoris are used as test organisms. For use in the confirmative and completed tests the usual selection of test bacteria and strains, which have been rendered resistant to a certain antibiotic, may be used.


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