The presence of cytoplasmic lipid droplets is not sufficient to account for neutral lipid signals in the 1H spectra of neutrophils

✍ Scribed by G. L. May; K. Sztelma; T. C. Sorrell

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 605 KB
• Volume: 31
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910310215

✦ Synopsis

Abstract

Stimulation of human peripheral blood neutrophils with lipopolysaccharide (LPS), arachidonic acid (AA) and oleic acid (OA) resulted in significant increases in cytoplasmic lipid droplets. This phenomenon was also observed in enucleated and degranulated cytoplasts prepared from neutrophils stimulated with LPS. In contrast, only LPS and high concentrations of OA (10 μ__M__) produced an increase in the lipid intensities of the MR spectra of neutrophils as determined by COSY cross peak volume measurements. Lipid intensities in cells stimulated with OA (2.5 μ__M__) and AA (2.5 μ__M__) and phorbol myristate acetate (20 n__M__) were not elevated. LPS stimulation of resting cytoplasts resulted in increased lipid droplets but not MR lipid intensities. These data suggest that while cytoplasmic lipid droplets may correlate with MR lipid intensity under some circumstances, their presence is not sufficient to account for increased neutral lipid signals.