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The presence and orientation of ecto-5'-nucleotidase in rat liver lysosomes

✍ Scribed by Gerald A. Maguire; J.Paul Luzio


Book ID
115916643
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
444 KB
Volume
180
Category
Article
ISSN
0014-5793

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5'-Nucleotidase has been purified from rat glioblastoma cells (Rugli cells). The enzyme has been solubilized from plasma membranes by using Triton X-100 and CHAPS. Two affinity chromatographies on concanavalin A and 5'-AMP-Sepharose render the purified enzyme with a high specific activity (76.36 mum