The preparation of tightly coupled membrane vesicles from Paracoccus denitrificans

✍ Scribed by Wright W. Nichols; W.Allan Hamilton

• Book ID: 102626168
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 336 KB
• Volume: 87
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90572-9

✦ Synopsis

Nonequilibrium

sedimentation of membrane vesicles from Paracoccus denitrificans through Ficoll gradients results in a separation into two fractions. The fraction which pellets through dense Ficoll is uncoupled. The second fraction, retarded by dense Ficoll, shows both improved concentrative transport activity and greater uncoupler stimulation of respiration as compared to the original vesicle preparation.