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The preparation of pure salt-free nicotinamide coenzymes

✍ Scribed by F.M. Dickinson; P.C. Engel

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
478 KB
Volume
82
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple procedure is described for preparing NAD+, NADP+, and their analogs in a salt-free chromatographically pure state. The previous standard method of purification by elution from DEAE-cellulose with a phosphate gradient is unsatisfactory for this purpose, because subsequent gel filtration does not separate the coenzymes from phosphate. Good results are obtained, however, with a gradient of sodium chloride, which can be adequately separated from nucleotide coenzymes on Sephadex G-10. NAD+ prepared by this procedure, lyophilized, and stored for 3 months showed negligible deterioration. The problem of peak splitting of NAD+ and NADPe in phosphate on Sephadex G-10 is discussed in relation to recent similar observations with ATP and formate [

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Conformational energy calculations for d
Conformational energy calculations for dinucleotide molecules: A study of the nucleotide coenzyme nicotinamide adenine dinucleotide (NAD+)
✍ Janet M. Thornton; Peter M. Bayley πŸ“‚ Article πŸ“… 1977 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 785 KB

## Abstract A study of the conformational states of the dinucleotide coenzyme NAD^+^ has been made using semiempirical energy calculations. Taking low‐energy mononucleotide structures as starting conformations, energy minimizations have been performed. The lowest energy states are stacked structure