The preparation and properties of two new chromogenic substrates of trypsin

✍ Scribed by Bernard F. Erlanger; Nicholas Kokowsky; William Cohen

• Book ID: 118843367
• Publisher: Elsevier Science
• Year: 1961
• Tongue: English
• Weight: 685 KB
• Volume: 95
• Category: Article
• ISSN: 0003-9861
• DOI: 10.1016/0003-9861(61)90145-x

✦ Synopsis

The synthesis and properties of l-lysine p-nitroanilide dihydrobromide and benzoyl dl-arginine p-nitroanilide hydrochloride are described. Both compounds are hydrolyzed by trypsin, the latter being a substrate at least as sensitive as benzoyl l-argininamide. Their hydrolysis can be followed conveniently by colorimetric procedures, since one of the products, p-nitroaniline, is yellow. Values of K
m and k
3 for their reaction with trypsin were determined, as well as the K
4 of benzoyl d-arginine p-nitroanilide, which was isolated from a tryptic digest of the dl isomer. The pH-activity curves were also determined, that of l-LPA being in a more alkaline region than normally found for trypsin substrates. The possible significance of this shift is discussed.
Preliminary studies indicate that benzoyl dl-arginine p-nitroanilide hydrochloride is also hydrolyzed by papain.