The potentiometric stripping analysis of proteins

✍ Scribed by Michael J. Honeychurch; Michael J. Ridd

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 783 KB
• Volume: 8
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140080710

✦ Synopsis

Abstract

Ten proteins were analyzed by potentiometric stripping analysis (PSA) following reductive accumulation on a HMDE and oxidation with dissolved oxygen. During the reductive accumulation period the surface disulfide bonds are reduced to sulfhydryl pairs. The rate of oxidation of the sulfhydryl pairs is determined by the transport of dissolved oxygen to the electrode surface. Comparison of the PSA response to that obtained by constant current chronopotentiometry (CCCP) of the reduced protein in a deoxygenated solution showed that the presence of dissolved oxygen, in the PSA experiments stabilized the proteins from further conformational changes following the reduction of the surface disulfide bonds.